Finding Enzymes

Click here to pull in the data for this tutorial. The sequence pNEBR will be added to a project called Training and opened for you.

1. Open the Digests panel by clicking the scissors icon at the right.

   

2. The search box allows finding enzymes by name or number of cuts. Enter “2” to show all double cutters or enter “EcoRI” to pull it up.

   

3. Hover over the enzyme to see buffer information, and to jump to the cut site on the sequence.

4. Click on the EcoRI row to select it. Notice that the cut site on the sequence map turns colored. Click on it again to unselect it.

   

5. You can also filter enzymes by where they cut on the sequence. Click on the “multiple cloning site (ApaI-StuI)” annotation on the sequence map to select it. Click “anywhere except the current selection” at the bottom of the restriction panel and note that EcoRI is no longer a search result.

   

6. Clicking the scissors icon again to close the Digests panel. Double-click on a cut site on the sequence map, and notice that the panel automatically opens to show more information about the enzyme.